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SPECIAL ASPECTS OF DARK-INDUCED LEAF SENESCENCE IN A DOUBLE ARABIDOPSIS THALIANA KNOCK-OUT MUTANT GDH1GDH2
Original title
ОСОБЕННОСТИ ИНДУЦИРОВАННОГО СТАРЕНИЯ ЛИСТЬЕВ У ДВОЙНОГО НОКАУТ-МУТАНТА ARABIDOPSIS THALIANA ПО ГЕНАМ ГЛУТАМАТДЕГИДРОГЕНАЗЫ
Authors
A. A. Vlasova1, E. Yu. Garnik2, V. I. Belkov2, V. I. Tarasenko2, Yu. M. Konstantinov2
Contact information
1 Irkutsk State University, Irkutsk, Russia, Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.
2 Siberian Institute of Plant Physiology and Biochemistry SB RAS, Irkutsk, Russia, Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.
Pages
25-26
DOI
10.31255/978-5-94797-318-1-25-26
Abstract
Glutamate dehydrogenase (GDH, EC 1.4.1.2) catalyzes the oxidative deamination of glutamate to 2-oxoglutarate. GDH is encoded in Arabidopsis thaliana by three genes, but the major activity is provided by products of two of them, GDH1 and GDH2. We detected that the double knock-out Arabidopsis mutant gdh1gdh2 retains a green color of its leaves when incubated in the dark during four days and more, whereas wild type Arabidopsis plants turn yellow in the same conditions. We studied an expression of chlorophyll catabolism genes NYC1, PAO, PPH, SGR1, SGR2 and SGRL in green, yellowing and yellow leaves of the wild type Arabidopsis line Col-0 and in typical non-yellowing gdh1gdh2 leaves after 5-7 days in the dark. It was found that the expression of genes NYC1, PAO and SGR2 is enhanced in the Col-0 yellowing leaves, but not in the gdh1gdh2 leaves. It suggests that the genetic program of chlorophyll degradation is disrupted in the gdh1gdh2 mutant plants.