OPTIMIZATION OF THE NUTRIENT MEDIUM WHILE BINDING NEW VARIETY GRAPES IN VITRO

Original title

ОПТИМИЗАЦИЯ ПИТАТЕЛЬНОЙ СРЕДЫ ПРИ РАЗМНОЖЕНИИ НОВЫХ СОРТОВ ВИНОГРАДА IN VITRO

Authors

M.S. Batukaev^1,2, M.G. Shishkhaeva², A.A. Batukaev^1,2

Contact information

¹The Chechen State University, Grozny, Russia, Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.

²Chechen research Institute of agricultural, Grozny, Russia

Pages

1176-1179

DOI

10.31255/978-5-94797-319-8-1176-1179

Abstract

The main objective aim of study was to improve the technology of clonal micropropagation using growth regulators. There were included in experiment the following varieties: Augustine, Moldova, Vostorg, Muscat Italian, Early Magaracha, Gift of Magaracha, Viorica. Auxins and cytokinins in various concentrations and combinations were added into the culture medium as growth regulators. The conducted experiments showed the shoots regeneration of isolated apexes occurred at all concentrations of 6-BAP except additive formulation in an amount of 5.0 mg/l when the tops immediately began to blacken and died. Effective influence of 6-BAP concentrations was in the range of 0.5...1.0 mg/l. Nevertheless, the greatest increase of microshoots fixed in the embodiment with a concentration of 1.0 mg/l should be noted. To speed up the process of microshoots lengthening, the study of the gibberellic acid effect in different concentrations combined with 6-BAP was simultaneously conducted. The experience has shown that the combination of 0.5 mg/l 6-BAP+1.0 mg/l (GA) achieved the best result.